Send an Email for free membership
~:~:~:~:~:~:~:~:~:~:~:~:~:~:~:~:~:
>>>>> Help ME Circle <<<<
>>>> 27 January 2012 <<<<
Editorship : j.van.roijen@chello.nl
~:~:~:~:~:~:~:~:~:~:~:~:~:~:~:~:~:
American Society For Microbiology
Journal of Virology
J. Virol. JVI.06717-11;
published ahead of print 11 January 2012,
Detection of Murine Leukemia Virus in the
Epstein-Barr virus-positive human B-cell line
JY using a computational RNA-seq based
exogenous agent detection pipeline,
PARSES.
Zhen Lin1, Adriane Puetter1, Joseph Coco2,
Guorong Xu2, Michael J. Strong1, Xia Wang1,
Claire Fewell1, Melody Baddoo1, Christopher
Taylor2 and Erik K. Flemington1,*
Author Affiliations
1Tulane University Health Sciences Center and Tulane
Cancer Center, 1430 Tulane Avenue, SL79, New Orleans,
LA 70112
2University of New Orleans, 2000 Lakeshore Drive, New
Orleans LA 70148
ABSTRACT
Many cell lines commonly used for biological studies have
been found to harbor exogenous agents such as the human
tumor viruses, Epstein-Barr virus (EBV) and human
papillomavirus.
Nevertheless, broad-based, unbiased approaches to globally
assess the presence of ectopic organisms within cell model
systems has not previously been available.
We reasoned that high-throughput sequencing should
provide unparalleled insights into the microbiomes of tissue
culture cell systems.
Here we have used our RNA-seq analysis pipeline, PARSES
(Pipeline for Analysis of RNA-Seq Exogenous Sequences),
to investigate the presence of ectopic organisms within two
EBV-positive B-cell lines commonly used by EBV
researchers.
Sequencing datasets from both the Akata and JY B-cell
lines were found to contain reads for EBV, and the JY
dataset was found to also contain reads from the murine
leukemia virus (MuLV).
Further investigation revealed that MuLV transcription in JY
cells is highly active.
We also identified a number of MuLV alternative splicing
events and we uncovered evidence of
APOBEC3G-dependent DNA-editing.
Finally, RT-PCR analysis showed the presence of MuLV in
three other human B-cell lines (DG75, Ramos, and P3HR1
Cl. 13) commonly used by investigators in the Epstein-Barr
virus field.
We believe that a thorough examination of tissue culture
microbiomes using RNA-seq/PARSES-like approaches is
critical for the appropriate utilization of these systems in
biological studies.
FOOTNOTES
*Corresponding author: Tel: 504-988-1167, email:
eflemin@tulane.edu
Copyright =A9 2012, American Society for Microbiology. All
Rights Reserved.
Full text: http://bit.ly/Ab4Xv3
~~~~
---------------------------------------------
Send posts to CO-CURE@listserv.nodak.edu
Unsubscribe at http://www.co-cure.org/unsub.htm
---------------------------------------------
Co-Cure's purpose is to provide information from across the spectrum of
opinion concerning medical, research and political aspects of ME/CFS and/or
FMS. We take no position on the validity of any specific scientific or
political opinion expressed in Co-Cure posts, and we urge readers to
research the various opinions available before assuming any one
interpretation is definitive. The Co-Cure website <www.co-cure.org> has a
link to our complete archive of posts as well as articles of central
importance to the issues of our community.
---------------------------------------------
