Analysis of single nucleotide polymorphisms in XMRV patient-derived
integration sites reveals contamination from cell lines acutely
infected by XMRV
Alice Rusmevichientong1, Jaydip Das Gupta2, Petra S. Elias1, Robert H.
Silverman2, and Samson A. Chow1,*
1 Department of Molecular and Medical Pharmacology, and UCLA AIDS
Institute, UCLA School of Medicine, Los Angeles, California 90095
2 Department of Cancer Biology, Lerner Research Institute, Cleveland
Clinic, Cleveland, Ohio 44195
* Corresponding author. Mailing address: Department of Molecular and
Medical Pharmacology, UCLA School of Medicine, CHS 23-133, 605 Charles
E. Young Dr. South, Los Angeles, CA 90095, USA. Tel: (310) 825-9600.
Fax: (310) 825-6267. E-mail: schow@mednet.ucla.edu
ABSTRACT
We analyzed xenotropic murine leukemia virus-related virus (XMRV)
integration site sequences previously identified from human prostate
tissues for single nucleotide polymorphisms (SNPs) to discriminate
between patient and potential cell line sources of the proviruses. The
SNPs of two integration sites were identical to those in cell lines
but not the patients, whereas the data on the remaining 12 integration
sites were inconclusive. Our results provide direct evidence for
contamination during analysis of XMRV integration sites.
---------------------------------------------
Send posts to CO-CURE@listserv.nodak.edu
Unsubscribe at http://www.co-cure.org/unsub.htm
Co-Cure Archives: http://listserv.nodak.edu/archives/co-cure.html
---------------------------------------------
Co-Cure's purpose is to provide information from across the spectrum of
opinion concerning medical, research and political aspects of ME/CFS and/or
FMS. We take no position on the validity of any specific scientific or
political opinion expressed in Co-Cure posts, and we urge readers to
research the various opinions available before assuming any one
interpretation is definitive. The Co-Cure website <www.co-cure.org> has a
link to our complete archive of posts as well as articles of central
importance to the issues of our community.
---------------------------------------------
