Virology. 2011 Sep 16. [Epub ahead of print]
No detectable XMRV in subjects with chronic fatigue syndrome from Quebec.
Cool M, Bouchard N, Mass=E9 G, Lagani=E8re B, Dumont A, Hanna Z, Phaneuf
D, Morisset R, Jolicoeur P.
SourceLaboratory of Molecular Biology, Clinical Research Institute of
Montreal, 110 Pine Avenue West, Montreal, Quebec, Canada H2W 1R7.
Abstract
We investigated the presence of XMRV in a cohort of Quebec patients
with chronic fatigue syndrome (CFS). DNA was purified from activated
peripheral blood mononuclear cells (PBMCs) and PCR was used to detect
XMRV gag and env in 72 patients. Anti-XMRV antibodies were searched in
sera of 62 patients by Western blot analysis. Attempts to detect XMRV
antigens was made, using immunofluorescence with Gag anti-p30
antiserum on activated PBMC from 50 patients. Plasma viremia was
measured by RT-PCR on 9 subjects. Finally, detection of infectious
virus in 113 CFS subjects was made by co-culture of PHA+IL-2 activated
PBMC with human LNCaP carcinoma cells, and by infecting the same
susceptible cells with plasma, using a reverse transcriptase (RT)
assay as a readout in both experiments. No detection of XMRV
footprints nor infectious virus was detected with any of the
approaches, in any of the tested individuals.
Copyright =A9 2011 Elsevier Inc. All rights reserved.
PMID:21925693[PubMed - as supplied by publisher]
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