Preprint
Date: September 24, 2011
URL: http://www.virologyj.com
http://7thspace.com/headlines/395041/xenotropic_murine_leukemia_virus_related_virus_is_not_associated_with_chronic_fatigue_syndrome_in_patients_from_different_areas_of_the_us_in_the_1990s.html
Xenotropic Murine Leukemia Virus-Related Virus is not associated
with Chronic Fatigue Syndrome in patients from different areas of
the US in the 1990s
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Mir Ali, Janet Dale, Christine Kozak, Raphaela Goldbach-Mansky,
Frederick Miller, Stephen Straus, Jeffrey Cohen
Abstract
In 2009, xenotropic murine leukemia virus-related virus (XMRV) was
reported in 67% of patients with chronic fatigue syndrome (CFS)
compared to 4% of controls. Since then numerous reports failed to
detect XMRV in other cohorts of CFS patients, and some studies
suggested that XMRV sequences in human samples might be due to
contamination of these samples with mouse DNA.
Results
We determined the prevalence of XMRV in patients with CFS from similar
areas in the United States as the original 2009 study, along with
patients with chronic inflammatory disorders and healthy persons.
Using quantitative PCR, we initially detected very low level signals
for XMRV DNA in 15% of patients with CFS; however, the frequency of
PCR positivity was no different between patients with CFS and
controls. Repeated attempts to isolate PCR products from these
reactions were unsuccessful.
These findings were supported by our observations that PHA and IL-2
stimulation of peripheral blood mononuclear cells from patients with
apparently low levels of XMRV, which induced virus replication in the
2009 report, resulted in the disappearance of the signal for XMRV DNA
in the cells. Immunoprecipitation of XMRV-infected cell lysates using
serum from patients from whom we initially detected low levels of XMRV
DNA followed by immunoblotting with antibodies to XMRV gp70 protein
failed to detect antibody in the patients, although one control had a
weak level of reactivity.
Diverse murine leukemia virus (MLV) sequences were obtained by nested
PCR with a similar frequency in CFS patients and controls. Finally, we
did not detect XRMV sequences in patients with several chronic
inflammatory disorders including rheumatoid arthritis, Bechet's
disease, and systemic lupus erythematosus.
Conclusions
We found no definitive evidence for XMRV DNA sequences or antibody in
our cohort of CFS patients, which like the original 2009 study,
included patients from diverse regions of the United States.
In addition, XMRV was not detected in a cohort of patients with
chronic inflammatory disorders.
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(c) 2011 BioMedCentral
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