J Proteome Res. 2011 Jul 27. [Epub ahead of print]
Unraveling persistent host cell infection with Coxiella burnetii by
quantitative proteomics.
Vranakis I, De Bock PJ, Papadioti A, Samoilis G, Tselentis Y, Gevaert
K, Tsiotis G, Psaroulaki A.
Abstract
The interaction between the immune system and invading bacteria is
sufficient to eradicate microorganisms for the majority of bacterial
infections, but suppression of the microbicidal response leads to
reactivation or chronic evolution of infections and to bacterial
persistence. To identify the cellular pathways affected by bacterial
persistence, we applied the MS-driven COmbined FRActional DIagonal
Chromatography (COFRADIC) proteomics technique for a comparative study
of protein expression in the C. burnetii strains Nine Mile (NM) and
its respective strain (NMper) isolated from 18 months persistently
infected cell cultures. In total, three different proteome comparisons
were performed with the total bacterial proteome, potentially secreted
bacterial proteins and the eukaryotic infected proteome being
assessed. Our results revealed that among the 547 identified bacterial
proteins, 53 had significantly altered levels of expression
andindicated potential metabolic differences between the two strains.
Regarding differences in the secreted proteins between both strains
and different modulation of the host cell machineries reflect at least
large rearrangements of both bacterial and eukaryotic proteomes during
the persistent model of infection when compared to the acute one and
emphasizes that C. burnetii orchestrates a vast number of different
bacterial and eukaryotic host cell processes to persist within its
host.
PMID:21790200[PubMed - as supplied by publisher]
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