Diagn Microbiol Infect Dis. 2011 Jul 25. [Epub ahead of print]
Xenotropic murine leukemia virus-related virus in monozygotic twins
discordant for chronic fatigue syndrome.
Jerome KR, Diem K, Huang ML, Selke S, Corey L, Buchwald D.
Department of Laboratory Medicine, University of Washington, Seattle,
WA, USA; Vaccine and Infectious Disease Division, Fred Hutchinson
Cancer Research Center, Seattle, WA, USA.
Abstract
A recent report suggested an association between xenotropic murine
leukemia virus-related virus (XMRV) and chronic fatigue syndrome
(CFS). If confirmed, this would suggest that antiretroviral therapy
might benefit patients suffering from CFS. We validated a set of
assays for XMRV and evaluated the prevalence of XMRV in a cohort of
monozygotic twins discordant for CFS. Stored peripheral blood
mononuclear cell (PBMC) samples were tested with 3 separate polymerase
chain reaction (PCR) assays (one of which was nested) for XMRV DNA,
and serum/plasma was tested for XMRV RNA by reverse transcription
(RT)-PCR. None of the PBMC samples from the twins with CFS or their
unaffected co-twins was positive for XMRV, by any of the assays. One
plasma sample, from an unaffected co-twin, was reproducibly positive
by RT-PCR. However, serum from the same day was negative, as was a
follow-up plasma sample obtained 2 days after the positive specimen.
These data do not support an association of XMRV with CFS.
Copyright =A9 2011 Elsevier Inc. All rights reserved.
PMID:21795004[PubMed - as supplied by publisher]
---------------------------------------------
Send posts to CO-CURE@listserv.nodak.edu
Unsubscribe at http://www.co-cure.org/unsub.htm
Too much mail? Try a digest version. See http://www.co-cure.org/digest.htm
---------------------------------------------
Co-Cure's purpose is to provide information from across the spectrum of
opinion concerning medical, research and political aspects of ME/CFS and/or
FMS. We take no position on the validity of any specific scientific or
political opinion expressed in Co-Cure posts, and we urge readers to
research the various opinions available before assuming any one
interpretation is definitive. The Co-Cure website <www.co-cure.org> has a
link to our complete archive of posts as well as articles of central
importance to the issues of our community.
---------------------------------------------
