Transfusion. 2011 Aug 19. doi: 10.1111/j.1537-2995.2011.03267.x. [Epub
ahead of print]
Absence of xenotropic murine leukemia virus-related virus in blood
donors in China.
Mi Z, Lu Y, Zhang S, An X, Wang X, Chen B, Wang Q, Tong Y.
SourceFrom the Beijing Institute of Microbiology and Epidemiology and
Affiliated Hospital, Academy of Military Medical Sciences, Beijing,
China.
Abstract
BACKGROUND: Xenotropic murine leukemia virus-related virus (XMRV) is a
novel human gammaretrovirus that was first identified in patients with
prostate cancer in 2006. Subsequent studies have shown that XMRV is
also detected in patients with chronic fatigue syndrome (CFS) and even
in some healthy controls and blood donors. However, some conflicting
findings have been reported by different laboratories or in different
regions. The association of XMRV with human diseases and the
prevalence of XMRV in different populations needs to be further
determined.
STUDY DESIGN AND METHODS: XMRV was screened in 391 blood samples from
healthy blood donors in China. Nested reverse transcription-polymerase
chain reaction (PCR) was used to amplify gag and env genes of XMRV
from total RNA of peripheral blood mononuclear cells (PBMNCs) and
plasma, respectively. Quantitative PCR was performed to detect XMRV
env gene in genomic DNA of PBMNCs. To enhance the detection
sensitivity, plasma was added into LNCaP cells to amplify XMRV in the
plasma samples.
RESULTS: No XMRV was found in the 391 blood donors in China or in the
LNCaP cells inoculated with plasma from the blood donors.
CONCLUSION: Both PCR and virus isolation in highly permissive LNCaP
cells failed to detect XMRV in 391 Chinese blood donors, indicating
that XMRV infection might not be present in blood donors in China.
=A9 2011 American Association of Blood Banks.
PMID:21854397[PubMed - as supplied by publisher]
---------------------------------------------
Send posts to CO-CURE@listserv.nodak.edu
Unsubscribe at http://www.co-cure.org/unsub.htm
---------------------------------------------
Co-Cure's purpose is to provide information from across the spectrum of
opinion concerning medical, research and political aspects of ME/CFS and/or
FMS. We take no position on the validity of any specific scientific or
political opinion expressed in Co-Cure posts, and we urge readers to
research the various opinions available before assuming any one
interpretation is definitive. The Co-Cure website <www.co-cure.org> has a
link to our complete archive of posts as well as articles of central
importance to the issues of our community.
---------------------------------------------
