e.0023484
PLoS ONE 6(8): e23484. doi:10.1371/journal.pone.0023484
DNA Extraction Columns Contaminated with Murine Sequences
Otto Erlwein#, Mark J. Robinson#, Simon Dustan, Jonathan Weber, Steve
Kaye, Myra O. McClure*
Jefferiss Research Trust Laboratories, Section of Infectious Diseases,
Imperial College London, London, United Kingdom
Abstract
Sequences of the novel gammaretrovirus, xenotropic murine leukemia
virus-related virus (XMRV) have been described in human prostate
cancer tissue, although the amounts of DNA are low. Furthermore, XMRV
sequences and polytropic (p) murine leukemia viruses (MLVs) have been
reported in patients with chronic fatigue syndrome (CFS). In assessing
the prevalence of XMRV in prostate cancer tissue samples we discovered
that eluates from na=EFve DNA purification columns, when subjected to
PCR with primers designed to detect genomic mouse DNA contamination,
occasionally gave rise to amplification products. Further PCR
analysis, using primers to detect XMRV, revealed sequences derived
from XMRV and pMLVs from mouse and human DNA and DNA of unspecified
origin. Thus, DNA purification columns can present problems when used
to detect minute amounts of DNA targets by highly sensitive
amplification techniques.
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