eventually. Tom (@TomKindlon)]
http://download.abstractcentral.com/ACG2011/proofs/P283.html
P283
Oral Administration of the Probiotic Bifidobacterium Infantis 35624 to
Humans Induces Immunoregulatory Responses In Vivo
Eamonn Quigley, MD, FACG,1 David Groeger, BSc,2 Liam O'Mahony, PhD,3
John Bourke, MD, MRCPI,4 Paul Scully, PhD,1 Ted Dinan, MD, PhD,1
Fergus Shanahan, MD, FACG,1 Barry Kiely, PhD2 1. Alimentary
Pharmabiotic Centre, University College Cork, Cork, Ireland; 2.
Alimentary Health Ltd., Cork, Ireland; 3. Swiss Institute of Allergy
and Asthma Research (SIAF), University of Zurich, Davos, Switzerland;
4. Cork University Hospital, University College Cork, Cork, Ireland.
Purpose: The commensal microbiota is required for optimal host
development and ongoing immunological homeostasis, which involves an
inter-dependence between microbes and immunity. Microbial dysbiosis
has been proposed as one possible explanation for the increased
incidence of a wide range of inflammatory disorders, suggesting that
manipulation of the microbiota with appropriate microbes may promote
immune regulatory mechanisms and reduce aberrant inflammatory
activity. Bifidobacterium longum subsp. infantis 35624 (B. infantis)
is a commensal microbe which has been extensively studied in murine
models and shown to reduce intestinal and extra-intestinal
inflammatory responses, in part via the induction of Foxp3+ T
regulatory cells. The aim of this study was to determine if B.
infantis could influence systemic pro-inflammatory biomarkers in
patients with inflammatory disease.
Methods: Healthy human volunteers (n=22), and patients with psoriasis
(n=27), ulcerative colitis (n=24) and chronic fatigue syndrome (n=50)
were recruited into double-blind, placebo controlled studies whereby
individuals received approximately 109 - 1010 B. infantis live cells
per day or placebo product for 8 weeks. Plasma was obtained at week 0
and week 8. Cytokine and c-reactive protein (CRP) levels were
determined using ultra-sensitive detection kits on the mesoscale
discovery multiplex platform.
Results: Plasma levels of the anti-inflammatory cytokine, IL-10, were
significantly increased in healthy volunteers and psoriasis patients
fed B.
infantis, but not placebo, for 8 weeks. In contrast, plasma levels of
the pro-inflammatory cytokines TNF-alpha and IL-6 were significantly
reduced in all patient groups that were administered B. infantis. In
addition, associated with decreased pro-inflammatory cytokine levels,
plasma CRP levels were also significantly reduced in psoriasis,
ulcerative colitis and chronic fatigue syndrome patients at the end of
treatment with B. infantis compared to placebo treated patients.
Conclusion: The human immunological response to B. infantis further
supports the hypothesis that manipulation of the microbiota with
specific therapeutic microbes can have a significant effect on host
inflammatory processes. The anti-inflammatory effect is not restricted
to a specific disease state, suggesting that B. infantis induces a
critical cellular response, which may include the induction of
regulatory cell subsets.
Disclosure - Mr. David Groeger - Employee Alimentary Health Ltd. Dr.
Barry Kiely - Employee Alimentary Health Dr. Liam O'Mahony -
Consultant Alimentary Health Ltd. Prof. Fergus Shanahan - Consultant
Alimentary Health Ltd. Prof.
Eamonn M. Quigley - Consultant Alimentary Health Ltd. Prof. Timothy G. Dinan
- Consultant Alimentary Health LTd.
This research was supported by an industry grant from Alimentary Health Ltd.
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