evidence for extra-hepatic cells sustaining hepatitis C virus
penetration.
J Viral Hepat. 2011 Aug;18(8):562-70
Authors: B=FCrgel B, Friesland M, Koch A, Manns MP, Wedemeyer H,
Weissenborn K, Schulz-Schaeffer WJ, Pietschmann T, Steinmann E, Ciesek
S
Abstract
Patients with chronic hepatitis C virus (HCV) infection show an
increased incidence of nervous system disorders such as chronic
fatigue syndrome, depression and cognitive dysfunction. It is unclear
whether this is because of HCV replication in the brain and in
peripheral neuronal cells or to more indirect effects of HCV infection
on the central or peripheral nervous system.
The aim of this study was to investigate whether cells originating
from these tissues are permissive for HCV cell entry, RNA replication
and virus assembly.
Among eight cell lines analysed, the human peripheral neuroblastoma
cell line SKNMC expressed all HCV entry factors and was efficiently
infected with HCV pseudoparticles (HCVpp) independent of the HCV
genotype. All remaining cell types including human neuroblastoma and
glioblastoma cell lines and microglial cells lacked expression of at
least one host factor essential for HCV entry. When transfected with
HCV luciferase reporter virus RNA, inoculated with HCV reporter
viruses or challenged with high-titre cell culture-derived HCV, none
of these cells supported detectable HCV RNA replication.
Thus, in conclusion, this comprehensive screening did not reveal
evidence directly strengthening the notion that HCV enters and
replicates in the central nervous system.
However, productive viral entry into the peripheral neuroblastoma cell
line SKNMC indicates that HCV may penetrate into certain nonhepatic
(liver) cell types which may serve as viral reservoirs and could
modulate (regulate) viral pathogenesis.
PMID: 20579278 [PubMed - indexed for MEDLINE]
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