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>>>>> Help ME Circle <<<<
>>>> 4 August 2011 <<<<
Editorship : j.van.roijen@chello.nl
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http://imeassoc.com/New_XMRV_evidence.html
IMEA
INTERNATIONAL ME ASSOCIATION
New evidence: Human gamma retroviruses
have too large a sequence diversity to be a
lab contaminantXMRV is a colloquial name
for a xenotropic polytopicgamma-
retrovirus.
Polytropic and modified polytropic gammas have also
been detected in over 90% of patients with ME, but
asyet have no colloquial name.
A small group of retro virologists, including John
Coffin, Myra McClure and Greg Towers, are convinced
that these viruses cannot infect humans.
They have been attempting to prove their belief that
this discovery represents nothing but laboratory
contamination.
One of the gamma retroviruses discovered shows
very little sequence variation in one of its genes in
the three isolates sequenced thus far, but a
thousand positive findings have not yet been
fully sequenced.
This has been used as evidence that the virus is
the product of a lab experiment and perfectly
harmless, despite its proven ability to induce an
immune response in human subjects.
However, it now emerges that the virus detected in
some people is showing considerable sequence
variation, which the contamination belief cannot
account for.
The following sequence has now been entered into the
Genbank:
*Partial molecular cloning with novel consensus PCR
primers of the murine JHK retrovirus of human origin, a
variant of the Xenotropic murine leukemia virus-related
virus (XMRV)*: http://1.usa.gov/qGISo8
Xenotropic MuLV-related virus 5' LTR, partial sequence;
and gag protein (gag) gene, partial cds
GenBank: HM119591.1
Halligan,B.D., Sun,H.-Y., Cashdollar,L.W.,
Kushnaryov,V.M. and Grossberg,S.E.
This new sequence, along with the new complete proviral
genome, isolate S-162 (http://1.usa.gov/n9TgYW) from
Lithuania, the recent addition of sequences from the Bill
Switzer at the CDC (http://bit.ly/ozgzUa), and the
polytropic and modified polytriopic sequences from the
WPI (http://1.usa.gov/q9hMaB), greatly increases the
diversity of these viruses.
John Coffins study, 'Recombinant Origin of the Retrovirus
XMRV' (Paprotka, 2011), cannot explain human gamma
retrovirus sequences with variation of this magnitude.
The conclusions in Paprotka et al. are clearly wrong.
The paper needs investigating as glaring flaws in
methodology have already been revealed.
This easily explains why the people who set their assays
to detect VP-62 were unable to detect wild-type virus.
International ME Association
3rd August 2011
Disclaimer: This article is not intended to provide medical
advice, diagnosis or treatment.
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