Mol Cell Proteomics. 2011 Aug 4. [Epub ahead of print]
Profiling the humoral immune response of acute and chronic Q fever by
protein microarray.
Vigil A, Chen C, Jain A, Nakajima-Sasaki R, Jasinskas A, Pablo J,
Hendrix LR, Samuel JE, Felgner PL.
SourceUniversity of California, Irvine, United States;
Abstract
Antigen profiling using comprehensive protein microarrays is a
powerful tool for characterizing the humoral immune response to
infectious pathogens. Coxiella burnetii is a CDC category B
bioterrorist infectious agent with worldwide distribution. In order to
assess the antibody repertoire of acute and chronic Q fever patients
we have constructed a protein microarray containing 93% of the
proteome of Coxiella burnetii, the causative agent of Q fever. Here we
report the profile of the IgG and IgM seroreactivity in 25 acute Q
fever patients in longitudinal samples. We found that both early and
late time points of infection have a very consistent repertoire of IgM
and IgG response, with a limited number of proteins undergoing
increasing or decreasing seroreactivity. We also probed a large
collection of acute and chronic Q fever patient samples and identified
serological markers that can differentiate between the two disease
states. In this comparative analysis we confirmed the identity of
numerous IgG biomarkers of acute infection, identified novel IgG
biomarkers for acute and chronic infections, and profiled for the
first time the IgM antibody repertoire for both acute and chronic Q
fever. Using these results we were able to devise a test that can
distinguish acute from chronic Q fever. These results also provide a
unique perspective on isotype switch and demonstrate the utility of
protein microarrays for simultaneously examining the dynamic humoral
immune response against thousands of proteins from a large number of
patients. The results presented here identify novel seroreactive
antigens for the development of recombinant protein-based diagnostics
and subunit vaccines, and provide insight into the development of the
antibody response.
PMID:21817167[PubMed - as supplied by publisher]
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